Immunization with mannosylated peptide induces poor T cell effector functions despite enhanced antigen presentation
article
In this study, we investigated the development of T cell responses in mice after administration of a mannosylated ovalbumin peptide (M-OVA323-339). Immunization with M-OVA323-339 in complete adjuvant resulted in enhanced antigen presentation in draining lymph nodes. Monitoring the fate of CFSE-labeled ovalbumin peptide-specific TCR transgenic CD4+ T cells revealed that immunization with M-OVA323-339 induced normal clonal expansion, recirculation and CD62L expression of antigen-specific T cells in vivo. However, these T cells developed only poor effector functions, reflected by minimal IFN-γ production, low IgG2a levels in serum and poor peptide-specific delayed-type hypersensitivity (DTH) responses. This diminished inflammatory response was associated with decreased infiltration of T cell blasts and macrophages. Importantly, also mice with functional effector T cells did not mount a robust DTH response after a challenge with M-OVA323-339 in the ear, although their T cells responded normally to M-OVA323-339 in vitro. In conclusion, mannosylated peptide induces proliferation of T cells with impaired Th1 cell effector functions and additionally abrogates the activity of pre-existing effector T cells. © The Japanese Society for Immunology. 2007. All rights reserved.
Topics
C-type lectinsDelayed-type hypersensitivityImmune modulationTh1 immunityCD4 antigengamma interferonimmunoglobulin G2aL selectinovalbuminT lymphocyte receptoranimal cellanimal experimentanimal tissueantigen expressionantigen presentationantigen specificityantiinflammatory activityarticleCD4+ T lymphocytecell fatecellular immunityclonal variationcontrolled studycytokine productiondelayed hypersensitivityeffector cellfemaleimmunizationimmunoglobulin blood levelin vitro studyin vivo studylymph nodelymphoblastlymphocyte proliferationlymphocytic infiltrationmacrophagemolecular recognitionmousenonhumanpriority journalspleen cellT lymphocyteTh1 cellAmino Acid SequenceAnimalsAntigen PresentationFemaleHypersensitivity, DelayedImmunizationLymphocyte ActivationMannoseMiceMice, Inbred C57BLMice, TransgenicMolecular Sequence DataOvalbuminPeptidesReceptors, Antigen, T-CellTh1 Cells
TNO Identifier
240594
ISSN
09538178
Source
International Immunology, 20(1), pp. 117-127.
Pages
117-127
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