Quantitative analysis of Tenecteplase in rat plasma samples using LC-MS/MS as an alternative for ELISA

article




Buscher, B.A.P.

Gerritsen, H.

Schöll, I. van

Cnubben, N.H.P.

Brüll, L.P.
An LC-MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; MW 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and the specificity of the method. In addition, the developed method was applied to the quantitative analysis of Tenecteplase in rat plasma samples originating from a single-dose study in rats. © 2007 Elsevier B.V. All rights reserved.
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Analytical researchDigestionLiquid chromatographyMass spectrometryPlasmaProteinsQuantitative analysisBloodDigestive systemDrug productsEnzymesSensitivity analysisInternal standardTenecteplaseTrypsinChemical analysisTenecteplaseAnimal experimentArticleBlood samplingControlled studyDrug distributionEnzyme linked immunosorbent assayLiquid chromatographyMass spectrometryNonhumanPriority journalQuantitative analysisRatSingle drug doseAnimalsChromatography, LiquidEnzyme-Linked Immunosorbent AssayFibrinolytic AgentsRatsSensitivity and SpecificityTandem Mass SpectrometryTissue Plasminogen ActivatorRattus
TNO Identifier
240016
Repository link
https://resolver.tno.nl/uuid:8a8f41ec-3377-4b41-8d93-9f3ccd529b87
ISSN
15700232
Source
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 852(1-2), pp. 631-634.
Pages
631-634
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