Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis
article
A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in either feces or sera but induced the release of IFN-γ on restimulation of primed lymphocytes ex vivo. The results suggested that the protective efficacy provided by flagellin-expressing L. casei is mainly attributable to cell-mediated immune responses. In addition, an adjuvant-type effect of the antigen delivery system with L. casei was also observed. © 2007 Elsevier Ltd. All rights reserved.
Topics
BiotechnologyFlagellinLactobacillusSalmonellaVaccineAntibodyAntigenFlagellinGamma interferonRecombinant antigenAnimal experimentAnimal modelAnimal tissueAntibody specificityAntigen antibody complexAntigen expressionAntigen specificityBacterial infectionBacterial strainCellular immunityDrug efficacyEx vivo studyFeces analysisFemaleImmune responseImmunityImmunizationLactobacillus caseiLymphocyteMouseNonhumanPriority journalSalmonella entericaSalmonella enteritidisSalmonellosisSerumAdministration, OralAnimalsFemaleFlagellinHumansImmunizationInterferon Type IILactobacillus caseiLymphocyte ActivationMiceMice, Inbred C3HRecombination, GeneticSalmonella enteritidisSalmonella Infections, AnimalSalmonella Vaccines
TNO Identifier
239976
ISSN
0264410X
Source
Vaccine, 25(18), pp. 3599-3605.
Pages
3599-3605
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