Identification of outer membrane proteins of Yersinia pestis through biotinylation

article





Smither, S.J.

Hill, J.

Baar, B.L.M. van

Hulst, A.G.

Jong, A.L. de

Titball, R.W.
The outer membrane of Gram-negative bacteria contains proteins that might be good targets for vaccines, antimicrobials or detection systems. The identification of surface located proteins using traditional methods is often difficult. Yersinia pestis, the causative agent of plague, was labelled with biotin. Tagged proteins were visualised through streptavidin probing of Western blots. Seven biotinylated proteins of Y. pestis were identified including two porins and the putative virulence factor catalase peroxidase. Crown Copyright © 2006.
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BiotinylationOuter membraneProteomicsYersinia pestisBacterial enzymeBacterial proteinBiotinCatalaseCatalase peroxidaseOuter membrane proteinPeroxidaseStreptavidinArticleBacterial virulenceBiotinylationNonhumanPlaguePriority journalProtein analysisProteomicsWestern blottingYersinia pestisAmino Acid SequenceBacterial Outer Membrane ProteinsBiotinylationBlotting, WesternElectrophoresis, Gel, Two-DimensionalPlagueSpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationYersinia pestisNegibacteriaYersinia pestisBiotin, 58-85-5Catalase, 9001-05-2Peroxidase, 9003-99-0Streptavidin, 9013-20-1Bacterial Outer Membrane Proteins
TNO Identifier
239815
Repository link
https://resolver.tno.nl/uuid:97d4e568-e86d-41cc-a1de-0058271a8332
ISSN
0167-7012
Source
Journal of Microbiological Methods, 68(1 (January)), pp. 26-31.
Pages
26-31
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