Endogenous interleukin-10 protects against hepatic steatosis but does not improve insulin sensitivity during high-fat feeding in mice
article
Several studies have demonstrated an association in humans between plasma levels or production capacity of the antiinflammatory cytokine IL-10 and insulin sensitivity. The aim of our study was to investigate the protective role of endogenous IL-10 availability in the development of diet-induced insulin resistance. We compared parameters of glucose and lipid metabolism between IL-10-/- mice and wild-type (wt) mice fed a high-fat diet for 6 wk. This diet has previously been shown to induce steatosis and insulin resistance. After 6 wk on the high-fat diet, no differences in body weight, basal metabolism (measured by indirect calorimetry), or plasma levels of glucose, triglycerides, or cholesterol were observed between IL-10-/- and wt mice. Nonetheless, in IL-10-/- mice, plasma free fatty acid levels were 75% increased compared with wt mice after overnight fasting (P < 0.05). In addition, hepatic triglyceride content was 54% increased in IL-10-/- mice (P < 0.05). During a hyperinsulinemic euglycemic clamp, no differences were observed in whole-body or hepatic insulin sensitivity between both groups. We conclude that basal IL-10 production protects against hepatic steatosis but does not improve hepatic or whole-body insulin sensitivity, during high-fat feeding. Copyright © 2006 by The Endocrine Society. Chemicals / CAS: amyloid A protein, 59165-71-8; fibrinogen, 9001-32-5; protein kinase B, 148640-14-6; Blood Glucose; Dietary Fats; Fibrinogen, 9001-32-5; Glucose, 50-99-7; Interleukin-10, 130068-27-8; Lipids; Serum Amyloid A Protein
Topics
Biomedical ResearchAmyloid A proteinfibrinogeninterleukin 10protein kinase BAnimal experimentCalorimetryCholesterol blood levelControlled studyExperimental modelFatty liverFeedingGlucose blood levelHlucose metabolismHigh performance liquid chromatographyHyperinsulinemiaInsulin sensitivityLipid dietLipid metabolismMouseNonhumanPhosphorylationSteatosisTriacylglycerol blood levelAdipose TissueAnimalsBlood GlucoseChromatography, Thin LayerDietDietary FatsEnergy MetabolismFibrinogenGenotypeGlucoseGlucose Clamp TechniqueInsulin ResistanceInterleukin-10Lipid MetabolismLipidsLiver DiseasesMaleMiceMice, Inbred C57BLMice, KnockoutPhenotypeSerum Amyloid A Protein
TNO Identifier
239484
ISSN
00137227
Source
Endocrinology, 147(10), pp. 4553-4558.
Pages
4553-4558
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