Matrix metalloproteinase-2 is a consistent prognostic factor in gastric cancer
article
In a pioneer study, we showed 10 years ago that enhanced tissue levels of the matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in gastric cancers, as determined by zymography, were related with worse overall survival of the patients. To corroborate these observations, we now assessed MMP-2 and MMP-9 with new techniques in an expanded group of gastric cancer patients (n = 81) and included for comparison MMP-7, MMP-8 and the tissue inhibitors of MMPs, TIMP-1 and -2. All MMPs and TIMP-1 were significantly increased in tumour tissue compared to normal gastric mucosa. Matrix metalloproteinase-7, -8 and -9, and the TIMPs showed some correlations with the clinicopathologic parameters TNM, WHO and Laurén classification, but their levels were not related with survival. Regardless of the determination method used, that is, enzyme-linked immunosorbent assay or bioactivity assay, an enhanced tumour MMP-2 level did not show a significant correlation with any of the clinicopathological parameters, but was confirmed to be an independent prognostic factor in gastric cancer. © 2006 Cancer Research. Chemicals / CAS: gelatinase A, 146480-35-5; gelatinase B, 146480-36-6; matrilysin, 141256-52-2; tissue inhibitor of metalloproteinase 1, 140208-24-8; tissue inhibitor of metalloproteinase 2, 124861-55-8; Matrix Metalloproteinase 2, EC 3.4.24.24
Topics
Biomedical ResearchGelatinasesMMP-7MMP-8MMP-9TIMPTissue inhibitor of metalloproteinasesgelatinase Agelatinase Bmatrilysinneutrophil collagenasetissue inhibitor of metalloproteinase 1tissue inhibitor of metalloproteinase 2bioassaycancer classificationcancer patientcancer stagingcancer survivalcancer tissuecomparative studycontrolled studycorrelation analysisenzyme analysisenzyme assayenzyme linked immunosorbent assayhistopathologyhuman tissuemajor clinical studyprognosisprotein variantstomach cancerstomach mucosatissue sectionworld health organizationAdultAgedAged, 80 and overEnzyme-Linked Immunosorbent AssayFemaleHumansMaleMatrix Metalloproteinase 2Middle AgedPredictive Value of TestsPrognosisStomach NeoplasmsSurvival Analysis
TNO Identifier
239225
ISSN
00070920
Source
British Journal of Cancer, 94(7), pp. 1035-1040.
Pages
1035-1040