Human embryo-conditioned medium stimulates in vitro endometrial angiogenesis
article
Objective: Successful implantation and placentation depend on the interaction between the endometrium and the embryo. Angiogenesis is crucial at this time. In this article we investigate the direct influence of the human embryo on in vitro endometrial angiogenesis. Design: In vitro study. Setting: Human endometrial microvascular endothelial cells (hEMVEC) grown on an in vitro angiogenesis model. Intervention(s): Conditioned media (CM) of human embryos were used to stimulate in vitro angiogenesis. Main Outcome Measure(s): In vitro angiogenesis of hEMVEC. Result(s): Conditioned media of human embryos, containing significant amounts of vascular endothelial growth factor (VEGF)-A, as determined by enzyme-linked immunosorbent assay (ELISA), caused an increase in hEMVEC tube formation. This effect was prevented by soluble VEGF receptor 1, which quenches VEGF-A activity. Recombinant EGF alone and leukemia inhibitory factor in combination with VEGF-A stimulated hEMVEC tube formation. None of the other tested recombinant mediators, which have been described as produced by the early embryo/trophoblast (interleukin (IL) 10, transforming growth factor (TGF) β, placental growth factor, hCG, colony-stimulating factor 1, interferon-γ, insulin-like growth factor I and II, IL-6, platelet-derived growth factor, and TGFα), had an effect on tube formation by hEMVEC. Conclusion(s): For the first time, it is shown that the human embryo is able to stimulate in vitro endometrial angiogenesis at the time of implantation, a process that is mediated by VEGF-A. © 2006 American Society for Reproductive Medicine.
Topics
Biomedical ResearchEndometrial angiogenesisLeukemia inhibitory factorPlacental growth factorRecombinant chorionic gonadotropinRecombinant colony stimulating factor 1Recombinant epidermal growth factorRecombinant gamma interferonRecombinant interleukin 10Recombinant interleukin 6Recombinant platelet derived growth factorRecombinant somatomedin BRecombinant somatomedin CRecombinant transforming growth factor betaTransforming growth factor alphaVasculotropin AVasculotropin receptor 1AngiogenesisCell growthControlled studyCulture mediumDrug effectEmbryoEdometriumEndometrium cellEndothelium cellEnzyme linked immunosorbent assayFemaleHuman cellHuman embryoImplantationIn vitro studyPriority journalStimulationTrophoblastAngiogenic ProteinsCell Culture TechniquesCell ProliferationCell SurvivalCells, CulturedCulture Media, ConditionedNeovascularization, PhysiologicTissue Engineering
TNO Identifier
239201
ISSN
00150282
Source
Fertility and Sterility, 85(Suppl. 1), pp. 1232-1239.
Pages
1232-1239
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