Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates
article
Filamentous growth of Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of A. oryzae grown on wheat-based media were analysed using N-terminal sequence analysis. In a submerged wheat-based growth medium of A. oryzae, besides α-amylase, also an arabinosidase and xylanase were abundantly produced. In the extracts of A. oryzae grown on wheat-based solid substrate besides α-amylase and chitinase, two new proteins of 16 and 27 kDa were identified. These hypothetical proteins showed only close homologies to filamentous fungal proteins. © 2005 Elsevier B.V. All rights reserved.
Topics
BiotechnologyAspergillus oryzaeCereal substratesFilamentousCell cultureFungiSubstratesAspergillus oryzaeCereal substratesFilamentousEnzymesAlpha arabinofuranosidaseAmylaseChitinaseFungal proteinXylan endo 1,3 beta xylosidaseAmino terminal sequenceAspergillus oryzaeCerealCulture mediumFungus growthNonhumanPriority journalProtein analysisProtein secretionSequence homologyAspergillus oryzaeCerealsFungal ProteinsAspergillus oryzaeFungiTriticum aestivum
TNO Identifier
239134
ISSN
01681656
Source
Journal of Biotechnology, 121(4), pp. 482-485.
Pages
482-485
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