Matrix metalloproteinase 2 is associated with stable and matrix metalloproteinases 8 and 9 with vulnerable carotid atherosclerotic lesions: A study in human endarterectomy specimen pointing to a role for different extracellular matrix metalloproteinase inducer glycosylation forms
article
Background and Purpose - We studied matrix metalloproteinases (MMP) 2, 8, and 9 and extracellular matrix metalloproteinase inducer (EMMPRIN) levels in relation to carotid atherosclerotic plaque characteristics. Methods - Carotid atherosclerotic plaques (n=150) were stained and analyzed for the presence of collagen, smooth muscle cell (SMC), and macrophages. Adjacent segments were used to isolate total protein to assess MMP-2 and MMP-9 activities and gelatin breakdown, MMP-8 activity, and EMMPRIN levels. Results - Macrophage-rich lesions revealed higher MMP-8 and MMP-9 activities, whereas SMC-rich lesions showed higher MMP-2 activity. The levels of less glycosylated EMMPRIN-45kD were higher in SMC-rich lesions and lower in macrophage-rich plaques. EMMPRIN-45kD was associated with MMP-2 levels, whereas EMMPRIN-58kD was related to MMP-9 levels. Conclusions - MMP-2, MMP-8, and MMP-9 activities differed among carotid plaque phenotypes. Different EMMPRIN glycosylation forms are associated with either MMP-2 or MMP-9 activity, which suggests that EMMPRIN glycosylation may play a role in MMP regulation and plaque destabilization. © 2005 American Heart Association, Inc. Chemicals / CAS: collagen, 9007-34-5; gelatin, 9000-70-8; gelatinase A, 146480-35-5; gelatinase B, 146480-36-6; Antigens, CD147, 136894-56-9; Collagen, 9007-34-5; Matrix Metalloproteinase 2, EC 3.4.24.24; Matrix Metalloproteinase 8, EC 3.4.24.34; Matrix Metalloproteinase 9, EC 3.4.24.35
Topics
CollagenGelatinGelatinase AGelatinase BCarotid atherosclerosisEndarterectomyEnzyme activityGlycosylationHuman cellHuman tissueImmunohistochemistryMacrophageProtein contentProtein degradationSmooth muscle fiberAnalysis of varianceChemistryClinical trialCytologyEnzymologyExtracellular matrixGeneticsMetabolismMethodologyMulticenter studyPathologyPhysiologyAtherosclerosisCarotid arteriesCarotid artery plaqueMatrix metalloproteinasesAnalysis of VarianceAntigens, CD147Carotid Artery DiseasesCell LineCollagenEndarterectomyExtracellular MatrixGlycosylationHumansImmunohistochemistryInflammationMacrophagesMatrix Metalloproteinase 2Matrix Metalloproteinase 8Matrix Metalloproteinase 9Myocytes, Smooth MusclePhenotype
TNO Identifier
239062
ISSN
00392499
Source
Stroke, 37(1), pp. 235-239.
Pages
235-239