The RETINOBLASTOMA-RELATED gene regulates stem cell maintenance in Arabidopsis roots

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Wildwater, M.

Campilho, A.

Perez-Perez, J.M.

Heidstra, R.

Blilou, I.

Korthout, H.

Chatterjee, J.

Mariconti, L.

Gruissem, W.

Scheres, B.
The maintenance of stem cells in defined locations is crucial for all multicellular organisms. Although intrinsic factors and signals for stem cell fate have been identified in several species, it has remained unclear how these connect to the ability to reenter the cell cycle that is one of the defining properties of stem cells. We show that local reduction of expression of the RETINOBLASTOMA-RELATED (RBR) gene in Arabidopsis roots increases the amount of stem cells without affecting cell cycle duration in mitotically active cells. Conversely, induced RBR overexpression dissipates stem cells prior to arresting other mitotic cells. Overexpression of D cyclins, KIP-related proteins, and E2F factors also affects root stem cell pool size, and genetic interactions suggest that these factors function in a canonical RBR pathway to regulate somatic stem cells. Expression analysis and genetic interactions position RBR-mediated regulation of the stem cell state downstream of the patterning gene SCARECROW. ©2005 Elsevier Inc. Molecular Sequence Numbers: GENBANK: AF245395;
Chemicals / CAS: cyclin D, 146587-97-5; Arabidopsis Proteins; RBR1 protein, Arabidopsis; Retinoblastoma Protein
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Biomedical Researchcyclin Dprotein p57retinoblastoma proteintranscription factor E2F1Arabidopsisarticlecell cyclecell sizegene controlgene expressiongene expression regulationgene functiongene interactiongene overexpressionmitosis indexmitosis inhibitionmitosis ratenucleotide sequenceplant parenchyma cellplant rootpriority journalprotein synthesis regulationsomatic celltumor suppressor geneArabidopsisArabidopsis ProteinsCell DifferentiationDown-RegulationPlant RootsRetinoblastoma ProteinSignal TransductionStem CellsArabidopsis
TNO Identifier
239044
Repository link
https://resolver.tno.nl/uuid:a8d4fa71-7661-4a67-bc38-928ae4f33d7c
ISSN
00928674
Source
Cell, 123(7), pp. 1337-1349.
Pages
1337-1349
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