Comprehensive two-dimensional liquid chromatography with on-line Fourier-transform-infrared-spectroscopy detection for the characterization of copolymers
article
The on-line coupling of comprehensive two-dimensional liquid chromatography (liquid chromatography × size-exclusion chromatography, LC × SEC) and infrared (IR) spectroscopy has been realized by means of an IR flow cell. The system has been assessed by the functional-group analysis of a series of styrene-methylacrylate (SMA) copolymers with varying styrene content. Ultraviolet (UV) detection was used as a detection technique to verify the detection with IR. The LC × SEC-IR functional-group contour plots (comprehensive chromatograms) obtained for styrene were in agreement with the contour plots constructed from the UV signal. In addition, contour plots can be obtained from non-UV-active groups. One such plot, for the carbonyl-stretching vibration of methylacrylate (MA), is shown. Selective detection of MA proved possible using flow cell IR detection. The combination of the contour plots for styrene and MA allowed a full characterization of the copolymer and it was revealed that the present series of SMA copolymers exhibited homogeneous chemical-composition distributions (CCDs). In addition, commercially available fast-SEC columns have been assessed in this study with respect to their potential to serve as second-dimension separation columns. © 2005 Elsevier B.V. All rights reserved.
Topics
Analytical researchCopolymersFlow cellFourier-transform- infrared-spectroscopyPolymer analysisSize-exclusion chromatographySolvent-elimination interfacesTwo-dimensional liquid chromatographyCharacterizationCopolymersFourier transform infrared spectroscopyInterfaces (materials)Size exclusion chromatographyStyreneUltraviolet radiationFlow cellPolymer analysisSolvent-elimination interfacesTwo-dimensional liquid chromatographyLiquid chromatographyCopolymerMethacrylic acidStyreneChemical compositionGel permeation chromatographyInfrared spectroscopyLiquid chromatographyPriority journalSeparation techniqueUltraviolet radiationVibrationAcrylatesChromatography, LiquidPolymersReproducibility of ResultsSensitivity and SpecificitySolventsSpectroscopy, Fourier Transform InfraredStyrenes
TNO Identifier
239020
ISSN
00219673
Source
Journal of Chromatography A, 1098(1-2), pp. 104-110.
Pages
104-110
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