The solvent-tolerant Pseudomonas putida S12 as host for the production of cinnamic acid from glucose
article
A Pseudomonas putida S12 strain was constructed that efficiently produced thefine chemical cinnamic acid from glucose or glycerol via the central metabolite phenylalanine. The gene encoding phenylalanine ammonia lyase from the yeast Rhodosporidium toruloides was introduced. Phenylalanine availability was the main bottleneck in cinnamic acid production, which could not be overcome by the overexpressing enzymes of the phenylalanine biosynthesis pathway. A successful approach in abolishing this limitation was the generation of a bank of random mutants and selection on the toxic phenylalanine anti-metabolite m-fluoro-phenylalanine. Following high-throughput screening, a mutant strain was obtained that, under optimised culture conditions, accumulated over 5 mM of cinnamic acid with a yield (Cmol%) of 6.7%. © Springer-Verlag 2005.
Topics
BiotechnologyBiosynthesisEnzymesGenesGlucoseGlycerolMetabolismOrganic acidsToxic materialsCinnamic acidPhenylalanine ammonia lyasePseudomonas putidaRhodosporidium toruloidesStrain3 fluorophenylalanineAntimetaboliteCinnamic acidGlucoseGlycerolPhenylalaninePhenylalanine ammonia lyaseSolventAmino acid synthesisBacterial strainBacterium cultureBacterium mutantBioaccumulationBioavailabilityControlled studyGene constructGene overexpressionGenetic codeHigh throughput screeningHostMetaboliteNonhumanPseudomonas putidaQuantum yieldRandomizationRhodosporidium toruloidesYeast3-Deoxy-7-Phosphoheptulonate SynthaseCatalysisCinnamatesDrug ToleranceFermentationGenetic EngineeringGenetic VectorsGlucoseMutationPhenylalaninePhenylalanine Ammonia-LyasePrephenate DehydratasePseudomonas putidaSolventsBacteria (microorganisms)Pseudomonas putidaRhodosporidium toruloides
TNO Identifier
238792
ISSN
01757598
Source
Applied Microbiology and Biotechnology, 69(2), pp. 170-177.
Pages
170-177
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