Characterisation of CwpA, a putative glycosylphosphatidylinositol-anchored cell wall mannoprotein in the filamentous fungus Aspergillus niger
article
Glycosylphosphatidylinositol (GPI)-anchored proteins in fungi are found at the cell surface, either as plasma membrane proteins (GPI-PMPs) or attached by a remnant of the GPI-anchor to the cell wall (GPI-CWPs). GPI-CWPs can be extracted from the cell wall by treatment with hydrofluoric acid (HF), which cleaves the phosphodiester bond that is present in the remnant of the GPI-anchor. The filamentous fungus Aspergillus niger contains at least seven HF-extractable cell wall mannoproteins. One gene encoding an HF-extractable cell wall mannoprotein, cwpA, was cloned and further characterised. The protein sequence of CwpA indicated the presence of two hydrophobic signal sequences both at the N-terminus and C-terminus of the protein, for entering the ER and the addition of a GPI-anchor, respectively. A CwpA-specific antiserum was raised and in combination with fractionation experiments, we show that this protein was abundantly present as an HF-extractable protein in the cell wall of A. niger. © 2005 Elsevier Inc. All rights reserved. Chemicals / CAS: hydrofluoric acid, 7664-39-3; DNA, Fungal; Fungal Proteins; Glycosylphosphatidylinositols; Hydrofluoric Acid, 7664-39-3; mannoproteins; Membrane Glycoproteins; Protein Sorting Signals
Topics
Biotechnologyω-siteCalcofluor WhiteCell wall integrityGlucanGPI-anchoringHF-extractionsHypersensitiveantiserumcell membrane proteinfungal proteinglycosylphosphatidylinositolhydrofluoric acidmannoproteinprotein CwpAunclassified drugamino acid sequenceamino terminal sequencearticleAspergillus nigercarboxy terminal sequencecell anchoragecell wallendoplasmic reticulumextractionfractionationfungal geneticsgenetic codehydrophobicitymolecular cloningnonhumannucleotide sequencepriority journalprotein analysisprotein localizationsignal transductionAmino Acid SequenceAspergillus nigerBase SequenceCell WallDNA, FungalFungal ProteinsGene DeletionGlycosylphosphatidylinositolsHydrofluoric AcidMembrane GlycoproteinsMolecular Sequence DataPhylogenyProtein Sorting SignalsSequence Analysis, DNAAspergillus nigerFungi
TNO Identifier
238741
ISSN
10871845
Source
Fungal Genetics and Biology, 42(10), pp. 873-885.
Pages
873-885
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