Detection of zilpaterol (Zilmax®) in calf urine and faeces with liquid chromatography-tandem mass spectrometry

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Hoof, N. van

Schilt, R.

Vlis, E. van der

Boshuis, P.

Baak, M. van

Draaijer, A.

Wasch, K. de

Wiele, M. van de

Hende, J.V. van

Courtheyn, D.

Brabander, H. de
Zilpaterol is a new powerful beta-agonist, which is officially registered for fattening purposes in cattle in Mexico and South Africa. Its chemical structure is different from the well-known beta-agonists. Therefore, the routinely used screening methods are not likely to be suited for the analysis of zilpaterol. Also gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry (LC-MS) methods need to be adapted to enable detection of zilpaterol. In this study, a LC-MS3 confirmatory method was developed for the simultaneous detection of zilpaterol and di-aromatic beta-agonists in urine samples. A LC-MS2 method was optimised for the detection of zilpaterol in faeces. To study the excretion profile in urine and faeces, a male veal calf was orally treated with daily doses of Zilmax ® during 2 weeks. Zilpaterol was mainly excreted via urine. © 2004 Elsevier B.V. All rights reserved.
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Analytical researchBeta-agonistsExcretion profileLC-MSnZilpaterolBody fluidsLiquid chromatographyMass spectrometryOptimizationCalf urineFaecesFatteningZilpaterolAlcoholsBeta adrenergic receptor stimulating agentUnclassified drugZilmaxZilpaterolAnimal experimentAnimal tissueCattleConference paperControlled studyDrug determinationDrug excretionDrug structureGas chromatographyLiquid chromatographyMaleMass spectrometryNonhumanPriority journalTandem mass spectrometryUrinalysisBos taurus
TNO Identifier
238312
Repository link
https://resolver.tno.nl/uuid:6b7a94db-d936-4b12-951d-2cd4d3ea074b
ISSN
0003-2670
Source
Analytica Chimica Acta, 529(1-2 SPEC. ISS.), pp. 189-197.
Pages
189-197
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