Expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori

article




Joosten, V.

Gouka, R.J.

Hondel, C.A.M.J.J. van den

Verrips, C.T.

Lokman, B.C.
We report the expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori. Fragments encoding VHHs were cloned in a suitable Aspergillus expression vector and transformants secreting VHH fragments were analysed for integrated gene copy-numbers, mRNA levels and protein production. Functional V HHs were detected in the culture medium, indicating the feasibility of producing this type of protein in a fungal expression system. Secreted V HHs were subjected to (extracellular) degradation, which could be partially prevented by the addition of BSA to the culture medium. © Springer-Verlag 2004.
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BiotechnologyCloningDegradationFunctionsFungiProteinsRNAVanadium compoundsAspergillus expressionHeavy-chain antibody fragmentsmRNA levelsAntibodiesAntibodyMessenger RNAProteinAntibodyAntibody productionAspergillus awamoriCulture mediumExpression vectorExtracellular spaceMolecular cloningNonhumanNucleotide sequenceProtein degradationProtein expressionAmino Acid SequenceAnimalsAspergillusCamelids, New WorldCloning, MolecularGene ExpressionImmunoglobulin Heavy ChainsImmunoglobulin Variable RegionMolecular Sequence DataRecombinant ProteinsSequence Homology, Amino AcidTransformation, GeneticAspergillusAspergillus awamoriLama glamavectors
TNO Identifier
238288
Repository link
https://resolver.tno.nl/uuid:d4d3e519-39ba-456a-8a07-39ea018f70ad
ISSN
01757598
Source
Applied Microbiology and Biotechnology, 66(4), pp. 384-392.
Pages
384-392
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