The flavones luteolin and apigenin inhibit in vitro antigen-specific proliferation and interferon-gamma production by murine and human autoimmune T cells
article
Plant-derived flavonoids are inhibitors of various intracellular processes, notably phosphorylation pathways, and potential inhibitors of cellular autoimmunity. In this study, the inhibiting effects of various flavonoids on antigen-specific proliferation and interferon-gamma (IFN-γ) production by human and murine autoreactive T cells were evaluated in vitro. T-cell responses were evaluated for the human autoantigen alpha B-crystallin, a candidate autoantigen in multiple sclerosis, and for the murine encephalitogen proteolipid protein peptide PLP (139-151). The flavones apigenin and luteolin were found to be strong inhibitors of both murine and human T-cell responses while fisitin, quercitin, morin and hesperitin, members of the subclasses of flavonoles and flavanones, were ineffective. Antigen-specific IFN-γ production was reduced more effectively by flavones than T-cell proliferation, suggesting that the intracellular pathway for IFN-γ production in T cells is particularly sensitive to flavone inhibition. These results indicate that flavones but not flavanoles or flavanones are effective inhibitors of the potentially pathogenic function of autoreactive T cells. The effects of flavones were the same for human and murine autoreactive T cells, stressing the usefulness of animal models of autoimmunity for further studies on the effects of flavonones on autoimmune diseases. © 2004 Elsevier Inc. All rights reserved. Chemicals / CAS: apigenin, 520-36-5; crystallin, 11046-99-4; gamma interferon, 82115-62-6; hesperetin, 520-33-2; luteolin, 491-70-3; morin, 480-16-0; quercetin, 117-39-5; antigen-specific helper factors; Apigenin, 520-36-5; Flavonoids; Interferon Type II, 82115-62-6; Luteolin, 491-70-3; Lymphokines
Topics
Apigenin
Autoimmune
EAE
experimental autoimmune encephalomyelitis
Flavonoids
IFN
IL
interferon
interleukin
Luteolin
MS
multiple sclerosis
PLP
proteolipid protein
T cells
crystallin
gamma interferon
morin
proteolipid protein
quercetin
animal cell
antigen specificity
autoimmunity
cell proliferation
cellular immunity
controlled study
cytokine production
drug efficacy
drug inhibition
evaluation
female
human cell
mouse
multiple sclerosis
nonhuman
Animals
Apigenin
Cell Survival
Dose-Response Relationship, Drug
Flavonoids
Humans
Interferon Type II
Luteolin
Lymphokines
Mice
T-Lymphocytes
TNO Identifier
237941
DOI
https://dx.doi.org/10.1016/j.bcp.2004.05.012
ISSN
00062952
Source
Biochemical Pharmacology, 68(4), pp. 621-629.
Pages
621-629
Files
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