Retrospective detection of exposure to sulfur mustard: Improvements on an assay for liquid chromatography-tandem mass spectrometry analysis of albumin/sulfur mustard adducts

article





Noort, D.

Fidder, A.

Hulst, A.G.

Woolfitt, A.R.

Ash, D.

Barr, J.R.
We here report on the further development of the method comprising the pronase digestion of albumin alkylated by sulfur mustard and the subsequent mass spectrometric analysis of an adducted tripeptide. This includes significant improvements in both the albumin isolation procedure and the automation of the microliquid chromatography-electrospray-tandem mass spectrometric analysis. We also report on the results of a small reference range study, in which we have established that there are no detectable interferences in sera from unexposed individuals.
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TripeptideAlkylationAutoanalysisBlood analysisChemical analysisChemical warfareControlled studyDigestionHumanHuman tissueLiquid chromatographyMass spectrometryProtein isolationSerumStandardAlbuminsAlkylationChemical warfare agentsChromatography, AffinityEnvironmental exposureHumansReference valuesRetrospective studiesMass spectrometryElectrospray ionizationMustard gas, 505-60-2Pronase, 9036-06-0Albumins
TNO Identifier
237891
Repository link
https://resolver.tno.nl/uuid:cd4fb3f1-3ce6-4312-9db4-3d8735c55807
ISSN
0146-4760
Source
Journal of Analytical Toxicology, 28(5), pp. 333-338.
Pages
333-338
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