Elevated levels of vascular endothelial growth factor in serum of patients with D+ HUS
article
The pathogenesis of hemolytic uremic syndrome (D+ HUS) is characterized by endothelial damage of glomeruli and tubules within the kidney. In several other diseases in which glomerular endothelial damage occurs, elevated serum levels of vascular endothelial growth factor (VEGF) have been reported. VEGF is involved in angiogenesis, permeabilization of blood vessel endothelium, and wound repair. In this study we evaluated VEGF levels in the serum of 40 D+ HUS patients in the acute phase and during the course of the disease. VEGF levels were measured using a double-sandwich ELISA. Indirect immunohistochemistry was performed for the detection of VEGF in renal biopsy material of 3 HUS patients. Significantly elevated VEGF levels were found in HUS patients compared with controls in both serum (P<0.001) and plasma (P<0.05). A significant relationship was found between VEGF levels and severity of the disease according to the classification of Gianantonio (P<0.05). Levels of VEGF in blood increased during the 2nd and 3rd week after HUS was diagnosed. Immunohistochemistry of renal biopsy material showed increased levels of the receptors for VEGF in the glomeruli. During the course of HUS, plasma VEGF levels increase and the increase is dependent on the severity of the disease. This is probably associated with the repair process. © IPNA 2004. Chemicals / CAS: vasculotropin receptor, 301253-48-5; vasculotropin, 127464-60-2; Receptors, Vascular Endothelial Growth Factor, EC 2.7.1.112; Vascular Endothelial Growth Factor A
Topics
Physiological SciencesD+ hemolytic uremic syndromeEndothelial damageFlt-1 and KDRRecovery phaseVascular endothelial growth factorvasculotropinvasculotropin receptorcontrolled studydisease classificationdisease coursedisease severityEnterobacter infectionenzyme linked immunosorbent assayglomerulushemolytic uremic syndromehuman tissueimmunohistochemistryinfantkidney biopsymajor clinical studyprotein blood leveltissue repairChildChild, PreschoolFemaleHemolytic-Uremic SyndromeHumansInfantKidneyMaleReceptors, Vascular Endothelial Growth FactorVascular Endothelial Growth Factor A
TNO Identifier
237867
ISSN
0931041X
Source
Pediatric Nephrology, 19(7), pp. 754-760.
Pages
754-760
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