Role of bacterial virulence proteins in Agrobacterium-mediated transformation of Aspergillus awamori

article



Michielse, C.B.

Ram, A.F.J.

Hooykaas, P.J.J.

Hondel, C.A.M.J.J. van den
The Agrobacterium-mediated transformation of Aspergillus awamori was optimized using defined co-cultivation conditions, which resulted in a reproducible and efficient transformation system. Optimal co-cultivation conditions were used to study the role of Agrobacterium tumefaciens virulence proteins in T-DNA transfer. This study revealed that inactivation of either of the regulatory proteins (VirA, VirG), any of the transport pore proteins (VirB), proteins involved in generation of the T-strand (VirD, VirC) or T-strand protection and targeting (VirE2) abolishes or severely reduces the formation of transformants. The results indicate that the Agrobacterium-mediated transformation of A. awamori requires an intact T-DNA machinery for efficient transformation; however, the plant host range factors, like VirE3, VirH, and VirF, are not important. Index descriptors: Agrobacterium tumefaciens; T-DNA; Virulence proteins; DNA-transformation; Aspergillus awamori; Filamentous fungi © 2004 Elsevier Inc. All rights reserved.
Topics













































BiotechnologyBacterial proteinProtein VirAProtein virCProtein virDProtein virGRegulator proteinUnclassified drugAspergillus awamoriBacterial virulenceBiotransformationControlled studyCultivarDNA strandDNA transferHost pathogen interactionNonhumanPlantPriority journalProtein targetingReaction optimizationReductionReproducibilityRhizobiumSystem analysisAspergillusBacterial ProteinsConjugation, GeneticDNA, BacterialDNA-Binding ProteinsGene DeletionGenes, BacterialIon ChannelsMutagenesis, InsertionalPlant Tumor-Inducing PlasmidsRhizobium radiobacterTemperatureTime FactorsTransformation, GeneticVirulence FactorsAgrobacteriumAgrobacterium tumefaciensAspergillusAspergillus awamoriBacteria (microorganisms)Viruses (infective agents)
TNO Identifier
237732
Repository link
https://resolver.tno.nl/uuid:b1421d17-9857-48ab-a329-5288f3f1f875
ISSN
10871845
Source
Fungal Genetics and Biology, 41(5), pp. 571-578.
Pages
571-578
Files
To receive the publication files, please send an e-mail request to TNO Repository.