Respiration-based measurement of lung deposition of a fluorescent dextrane aerosol in a marmoset monkey
article
A technique for measurement of respiration-based lung deposition of an aerosol was investigated and subsequently applied in a pilot study with a marmoset monkey. The technique consisted of an aerosol exposure system for a marmoset using a face mask and a previously constructed monkey chair, a method for recovery of fluorescent dextrane from lung material, and respiration measurement of the marmoset by whole-body plethysmography. In the pilot study, a ketamine-anesthetized marmoset was exposed for 20 min to an FITC-dextrane aerosol atmosphere (200 μg/L air, particle size 1.5 μm mass median aerodynamic diameter [MMAD]). It was found that 3.4% of the inhaled aerosol was deposited in the lungs; the aerosol was distributed over the lung lobes with an higher concentration at the distal side.
Topics
AerosolsAnimal experimentAnimal tissueAtmospheric dispersionBody burdenBody plethysmographyBreathingControlled studyEnvironmental exposureFace maskFluorescenceInhalationMarmosetMeasurementNonhumanParticle sizePilot studyTechniqueAnimalsCallithrixDose-response relationship, drugFluorescein-5-isothiocyanateLungMaleAnimaliaMarmosetsVertebrataDextran, 87915-38-6, 9014-78-2Dextrans, 9004-54-0Fluorescein isothiocyanate dextranFluorescein-5-isothiocyanate, 3326-32-7
TNO Identifier
237644
ISSN
08958378
Source
Inhalation Toxicology, 16(3), pp. 141-146.
Pages
141-146
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