Activation of the oxidative burst by yeast elictor in Catharanthus roseus cells occurs independently of the activation of genes involved in alkaloid biosynthesis
article
In Catharanthus roseus cell suspensions, expression of several terpenoid indole alkaloid (TIA) biosynthetic genes, including those encoding strictosidine synthase and tryptophan decarboxylase, is coordinately induced by fungal elicitors such as yeast extract (YE). This induction is mediated by several signaling steps including the biosynthesis of jasmonic acid, and the activation of the jasmonic acid-responsive ORCA transcription factors. We investigated a possible role of reactive oxygen species (ROS) as a second messenger in this system. YE was shown to activate the production of ROS, which was dependent on protein phosphorylation and calcium influx. However, ROS generation was neither necessary for the induction of genes involved in TIA biosynthesis by YE nor by itself sufficient to induce these genes. Therefore, we conclude that activation of the oxidative burst by YE occurs independently of the activation of genes involved in TIA biosynthesis. © 2004 Kluwer Academic Publishers.
Chemicals / CAS acetylcysteine, 616-91-1; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; calcium, 7440-70-2; gadolinium chloride, 10138-52-0; gadolinium, 7440-54-2; lanthanum chloride, 10099-58-8, 11098-96-7; lanthanum, 16096-89-2, 7439-91-0; nifedipine, 21829-25-4; tiron, 149-45-1, 149-46-2; Acetylcysteine, 616-91-1; Ascorbic Acid, 50-81-7; Calcium, 7440-70-2; Culture Media; gadolinium chloride, 10138-52-0; Gadolinium, 7440-54-2; lanthanum chloride, 10099-58-8; Lanthanum, 7439-91-0; Nifedipine, 21829-25-4; Reactive Oxygen Species; RNA, Plant; Secologanin Tryptamine Alkaloids; Tiron, 149-45-1
Chemicals / CAS acetylcysteine, 616-91-1; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; calcium, 7440-70-2; gadolinium chloride, 10138-52-0; gadolinium, 7440-54-2; lanthanum chloride, 10099-58-8, 11098-96-7; lanthanum, 16096-89-2, 7439-91-0; nifedipine, 21829-25-4; tiron, 149-45-1, 149-46-2; Acetylcysteine, 616-91-1; Ascorbic Acid, 50-81-7; Calcium, 7440-70-2; Culture Media; gadolinium chloride, 10138-52-0; Gadolinium, 7440-54-2; lanthanum chloride, 10099-58-8; Lanthanum, 7439-91-0; Nifedipine, 21829-25-4; Reactive Oxygen Species; RNA, Plant; Secologanin Tryptamine Alkaloids; Tiron, 149-45-1
Topics
TNO Identifier
43034
Source
Plant Molecular Biology, 55, pp. 797-805.
Pages
797-805
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