Simultaneous measurement of foveal spectral reflectance and cone-photoreceptor directionality

article



Zagers, N.P.A.

Kraats, J. van de

Berendschot, T.T.J.M.

Norren, D. van
An instrument for simultaneous measurement of foveal spectral reflectance and cone-photoreceptor directionality is described. The key element is an imaging spectrograph (spectral range of 420–790 nm) with its entrance slit conjugate to the pupil plane of a human eye. A 1.9-deg spot on the retina is sampled in 1 s. Video observation of the retina and the pupil facilitates proper alignment. Measurements were performed on 21 healthy subjects. Model analysis of the spectra provided densities of photostable ocular absorbers. As an example, macular pigment and melanin are discussed in more detail. Spatial profiles exhibited the optical Stiles–Crawford effect, reflecting cone-photoreceptor directionality. © 2002 Optical Society of America.
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biological modelinstrumentationmiddle agedphysiologyradiation scatteringretina coneretina foveaAdultAgedCones (Retina)FemaleFovea CentralisHumansMaleMiddle AgedModels, BiologicalOphthalmologyPupilScattering, RadiationImaging techniquesLight absorptionLight reflectionSpectrographsSpectrum analysisVideo recordingCone-photoreceptorsImage analysis
TNO Identifier
953593
Repository link
https://resolver.tno.nl/uuid:14a03392-7075-4f28-b68a-b323c67c6de2
ISSN
1559128X
Source
Applied Optics, 41(22), pp. 4686-4696.
Pages
4686-4696
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