Insertion of pea lectin into a phospholipid monolayer

article



Booij, P.

Demel, R.A.

Pater, B.S. de

Kijne, J.W.
Pea lectin (PSL) is a secretory sugar-binding protein, readily soluble in aqueous solutions of low osmolarity. However, PSL also appears to be associated with the plasma membrane at the tip of young pea root hairs. By using the Wilhelmy plate method, we found that PSL can insert into a lipid monolayer. This property appeared to be independent of the sugar- binding ability of the protein. This result suggests that PSL may be directly involved in membrane-mediated interactions with saccharide ligands, for example during root hair infection by symbiotic rhizobia. Chemicals/CAS: Lectins; pea lectin; Phospholipids; Plant Lectins
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MonolayerPea lectinPhosphatidylcholineWilhelmy plate methodPea lectinPhospholipidPlant lectinMetabolismPHProtein bindingCarbohydrate MetabolismCell MembraneHydrogen-Ion ConcentrationLectinsPhospholipidsPlant LectinsPlant RootsProtein BindingPisum sativum
TNO Identifier
280544
Repository link
https://resolver.tno.nl/uuid:438d4326-0764-46c8-b87e-ad3840c84f35
ISSN
01674412
Source
Plant Molecular Biology, 31(1), pp. 169-173.
Pages
169-173
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