Frequency of HPRT mutants in humans exposed to vinyl chloride via an environmental accident
article
The mutant frequency (MF) in the hypoxanthine-guanine-phosphoribosyl-transferase (HPRT) locus of peripheral blood T-lymphocytes was measured in a population environmentally exposed to vinyl chloride - a toxic and carcinogenic substance through an accidental release into the atmosphere. It was compared to MF in a control group of unexposed individuals. Both groups were re-investigated in a follow-up study, 2 years later. No significant difference could be observed in MF between exposed and controls either at the accident nor in the follow-up study. Approximately the same mean HPRT mutant frequencies were observed for both groups in T-lymphocytes from blood samples obtained shortly after the accident and from the follow-up blood samples. Both groups showed a higher mean MF in the re-investigation samples which is most probably due to the significantly lower average cloning efficiency (CE) under non-selective conditions and because of the inverse relationship between CE and MF. The exposed population showed a higher mean T-cell CE at the initial blood sampling as compared to the control group. The concurrent cytogenetic analyses of peripheral lymphocytes showed a significant increase in cells with aberrations in the exposed population. Clastogenic but not mutagenic activity of vinyl chloride was observed in our study. © 2001 Elsevier Science B.V. Chemicals/CAS: Air Pollutants; Hypoxanthine Phosphoribosyltransferase, EC 2.4.2.8; Mutagens; Vinyl Chloride, 75-01-4
Topics
Accidental environmental exposureHPRT-geneHuman T-lymphocytesIn vivoMutant frequencyVinyl chloridehypoxanthine phosphoribosyltransferasevinyl chlorideaccidentadultarticleblood samplingcontrolled studyenvironmental exposurefemalefollow upgenotoxicityhumanhuman cellhuman experimentmalemutation ratenormal humanpriority journalT lymphocyteAdultAir PollutantsClone CellsEnvironmental ExposureFemaleFollow-Up StudiesHumansHypoxanthine PhosphoribosyltransferaseMaleMiddle AgedMutagenesisMutagensMutationT-LymphocytesVinyl Chloride
TNO Identifier
280354
ISSN
13835718
Source
Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 494(1-2), pp. 87-96.
Pages
87-96
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