Matrix metalloproteinases-3, -8, -9 as markers of disease activity and joint damage progression in early rheumatoid arthritis
article
Objective: To analyse the relation between systemic levels of pro-MMP-3, -8, and -9 matrix metalloproteinase (MMP) activity in α2 macroglobulin (α2M)/MMP complexes and the progression of joint destruction in patients with recent onset rheumatoid arthritis (RA). Methods: 109 patients with RA of recent onset were entered into this longitudinal study. Patients were followed up for two years; clinical data, blood samples, and radiographs were obtained at baseline and at 1 and 2 years. Serum levels of MMPs were measured by sandwich ELISA and MMP activity assays. Results: During the two years joint damage progressed from 0 to 10 (median Sharp score, p<0.001). Stable levels of pro-MMP-3 and a significant decrease in the levels of pro-MMP-8 and -9 and α2M/MMP complexes were seen throughout the two years. Regression analysis showed that serum pro-MMP-3 levels at disease onset were independently associated with the progression of joint damage (B = 0.7, 95% CI 0.3 to 1.1, p = 0.001). Based on the rate of joint destruction, patients were divided into two subgroups: patients with mild and severe joint damage progression. The pro-MMP-3 levels were significantly higher in the group with severe compared with mild disease at all times. Levels of pro-MMP-8 and -9 were decreased in both groups, whereas α 2M/MMP complex levels decreased in the group with mild disease only. Conclusion: Serum levels of the MMPs studied are associated with disease activity, but serum pro-MMP-3 levels at the onset of disease are also predictive of joint damage progression. Chemicals/CAS: gelatinase B, 146480-36-6; stromelysin, 79955-99-0; alpha-Macroglobulins; Biological Markers; C-Reactive Protein, 9007-41-4; Matrix Metalloproteinase 3, EC 3.4.24.17; Matrix Metalloproteinase 9, EC 3.4.24.35; Matrix Metalloproteinases, EC 3.4.24.
Topics
Biomedical Researchgelatinase Bneutrophil collagenasestromelysinagedarthropathydisease activitydisease coursedisease markerenzyme activityenzyme blood levelenzyme linked immunosorbent assayfemalehumanjoint destructionlongitudinal studymajor clinical studymalepredictionrheumatoid arthritisAdultalpha-MacroglobulinsArthritis, RheumatoidBiological MarkersC-Reactive ProteinDisease ProgressionFemaleHumansJointsMaleMatrix Metalloproteinase 3Matrix Metalloproteinase 9Matrix MetalloproteinasesMiddle AgedProspective StudiesStatistics, Nonparametric
TNO Identifier
237374
ISSN
00034967
Source
Annals of the Rheumatic Diseases, 62(11), pp. 1094-1099.
Pages
1094-1099