Gene expression profiling of resting and activated vascular smooth muscle cells by serial analysis of gene expression and clustering analysis
article
Migration and proliferation of vascular smooth muscle cells (SMCs) are key events in atherosclerosis. However, little is known about alterations in gene expression upon transition of the quiescent, contractile SMC to the proliferative SMC. We performed serial analysis of gene expression (SAGE) of cultured, human SMCs, either grown under resting circumstances or activated with an atherogenic stimulus. Analysis of tags, representing 47,209 and 47,259 mRNAs from a library of resting and activated SMCs, respectively, identified 105 tags induced and 52 tags repressed greater than fivefold. To evaluate the relevance in SMC biology of unmatched, regulated tags, we performed hierarchical clustering analysis, based on their expression profiles in public SAGE databases, and clustered these novel genes in distinct groups. The regulation in SMCs was confirmed by Northern blotting for representative genes of these groups. Plasminogen activator inhibitor-2 has not been associated with atherosclerosis before and was localized to atherosclerotic lesions. © 2003 Elsevier Science (USA). All rights reserved.
Topics
Biomedical ResearchActivinCardiac ankyrin-repeat proteinConnective tissue growth factorPAI-2Serial analysis of gene expression (SAGE)SM22αSmooth muscle cellgene productmessenger RNAplasminogen activator inhibitor 2atherosclerosiscell activationcell culturecluster analysiscontrolled studycytologydata basegene expressiongene expression profilinggene librarygenetic analysishuman cellhuman tissueNorthern blottingvascular smooth muscleGene ExpressionGene Expression ProfilingGene LibraryHumansImmunohistochemistryMuscle, Smooth, VascularReverse Transcriptase Polymerase Chain ReactionRNA, MessengerUmbilical ArteriesSalvia
TNO Identifier
237243
ISSN
08887543
Source
Genomics, 82(3), pp. 288-299.
Pages
288-299
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