The most abundant protease inhibitor in potato tuber (cv. Elkana) is a serine protease inhibitor from the Kunitz family
article
The gene of the most abundant protease inhibitor in potato cv. Elkana was isolated and sequenced. The deduced amino acid sequence of this gene showed 98% identity with potato serine protease inhibitor (PSPI), a member of the Kunitz family. Therefore, the most abundant protease inhibitor was considered to be one of the isoforms of PSPI. The PSPI group represents ∼22% of the total amount of proteins in potato cv. Elkana and is composed of seven different isoforms that slightly differ in isoelectric point. Antibodies were raised against the two most abundant isoforms of PSPI. The binding of these antibodies to PSPI isoforms and protease inhibitors from different groups of protease inhibitor in potato showed that ∼70% of the protease inhibitors present in potato juice belong to the Kunitz family. Chemicals/CAS: Antibodies; Isoenzymes; Serine Proteinase Inhibitors.
Topics
Kunitz familyPotatoPotato serine protease inhibitorProtease inhibitorisoproteinplant extractpotato extractserine proteinase inhibitorunclassified drugamino acid sequenceantibody detectionbinding kineticsextractiongene isolationgene sequenceisoelectric pointnonhumanprotein familyproteinase inhibitionstrain identificationAmino Acid SequenceAntibodiesElectrophoresis, Polyacrylamide GelIsoenzymesMolecular Sequence DataProtein Structure, QuaternarySequence AlignmentSequence Analysis, DNASequence Analysis, ProteinSerine Proteinase InhibitorsSolanum tuberosumSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSolanum tuberosum
TNO Identifier
237223
ISSN
00218561
Source
Journal of Agricultural and Food Chemistry, 51(17), pp. 5001-5005.
Pages
5001-5005
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