Analysis of allelic expression patterns of IL-2, IL-3, IL-4, and IL-13 in human CD4+ T cell clones
article
The occurrence of monoallelic expression of cytokine genes in single cells has been convincingly demonstrated, but there have been few reports of this phenomenon in T cell clones. Here we describe studies on the expression of alleles of the human genes encoding IL-2, IL-3, IL-4, and IL-13 in human CD4+ T cell clones. In contrast to the results reported in mouse T cell clones and single human T cells, we found no evidence for the monoallelic expression of the IL-2, IL-3, and IL-13 genes. The gene for IL-4 showed an imbalance in expression from each allele, indicating differential expression of IL-4 alleles within or between IL-4-expressing cells. Chemicals/CAS: interleukin 13, 148157-34-0; interleukin 2, 85898-30-2; RNA, 63231-63-0; Interleukin-13; Interleukin-2; Interleukin-3; Interleukin-4, 207137-56-2; Interleukins.
Topics
AlleleCytokineExpressionT cellCD4 antigencytokineinterleukin 13interleukin 2interleukin 3interleukin 4RNAT lymphocyte receptor beta chainalleleallelic imbalanceanimal cellarticlecontrolled studygene expressiongene expression profilinggenetic analysisgenetic codegenotypeheterozygosityhumanhuman celllymphocyte clonemousenonhumanpriority journalreverse transcription polymerase chain reactionRNA isolationsingle nucleotide polymorphismT lymphocyteAllelesAnimalsBase SequenceCD4-Positive T-LymphocytesClone CellsGene ExpressionGenotypeHeterozygoteHumansInterleukin-13Interleukin-2Interleukin-3Interleukin-4InterleukinsMicePolymorphism, GeneticPolymorphism, Single Nucleotide
TNO Identifier
237209
ISSN
00142980
Source
European Journal of Immunology, 33(8), pp. 2142-2148.
Pages
2142-2148
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