A PCR-based method for identification of bifidobacteria from the human alimentary tract at the species level
article
A polymerase chain reaction (PCR)-based method was developed for the identification of isolates of Bifidobacterium at the species level. Using two Bifidobacterium-specific primers directed against the 16S ribosomal gene (Bif164 and Bif662), a PCR product was obtained from the type strains of 12 different bifidobacterial species that have been found in the human alimentary tract. After purification of the PCR products, the DNA was restricted with five different restriction enzymes. The size of the different restriction fragments was used as a fingerprint for the identification of individual bifidobacterial species. The amplified ribosomal DNA restriction analysis method was subsequently used to speciate bifidobacterial isolates from child's feces and from an in vitro model of the large intestine. © 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Topics
Physiological Sciences16S rDNAAlimentary tractAmplified ribosomal DNA restriction analysisBifidobacteriumBacterial DNABacterial enzymeBacterial proteinDNA 16SRestriction endonucleaseRibosome proteinAmplified ribosomal DNA restriction analysisBacterial strainBacterium identificationBacterium isolateBifidobacteriumControlled studyDigestive systemDNA fingerprintingFeces analysisHumanHuman tissueIn vitro studyLarge intestineNonhumanNucleotide sequencePolymerase chain reactionPriority journalRestriction fragmentBifidobacteriumChildDNA, BacterialFecesHumansIntestine, LargePolymerase Chain ReactionRestriction MappingRNA, Ribosomal, 16SBifidobacterium
TNO Identifier
237187
ISSN
03781097
Source
FEMS Microbiology Letters, 224(1), pp. 143-149.
Pages
143-149
Files
To receive the publication files, please send an e-mail request to TNO Repository.