Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori

article





Lokman, B.C.

Joosten, V.

Hovenkamp, J.

Gouka, R.J.

Verrips, C.T.

Hondel, C.A.M.J.J. van den
The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible endoxylanase promoter. Secretion of active ARP was achieved up to 800 mg l-1 in shake flask cultures. Western blot analysis showed that an rARP product of the correct molecular weight was produced. In contrast to several other studies about heterologous production of heme containing peroxidases, our results suggest that in A. awamori no heme limitation exists during overproduction of ARP. © 2003 Elsevier Science B.V. All rights reserved.
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BiotechnologyAspergillus awamoriHeme limitationHeterologous protein productionPeroxidaseCell cultureEnzyme kineticsFungiGrowth kineticsMolecular weightWestern blot analysisBiotechnologyFungal enzymePeroxidaseXylan endo 1,3 beta xylosidaseArthromyces ramosusAspergillus awamoriCulture mediumEnzyme analysisEnzyme synthesisFungusMolecular weightNonhumanPriority journalWestern blottingAspergillusBiotechnologyBlotting, NorthernBlotting, SouthernFermentationHemeMitosporic FungiPeroxidasePlasmidsPromoter Regions (Genetics)'Arthromyces ramosus'AspergillusAspergillus awamoriFungi
TNO Identifier
237151
Repository link
https://resolver.tno.nl/uuid:34159064-48be-4ce7-ae2c-58a97a277b68
ISSN
01681656
Source
Journal of Biotechnology, 103(2), pp. 183-190.
Pages
183-190
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