Characterisation of botulinum toxins type A and B, by matrix-assisted laser desorption ionisation and electrospray mass spectrometry
article
A method earlier developed for the mass spectrometric (MS) identification of tetanus toxin (TTx) was applied to botulinum toxins type A and B (BTxA and BTxB). Botulinum toxins are extremely neurotoxic bacterial toxins, likely to be used as biological warfare agent. Biologically active BTxA and BTxB are comprised of a protein complex of the respective neurotoxins with specific haemagglutinins (HAs) and non-toxic non-haemagglutinins (NTNHs). These protein complexes are also observed in mass spectrometric identification. The particular BTxA complex, from Clostridium botulinum strain 62A, almost completely matched database data derived from genetic sequences known for this strain. Although no such database information was available for BTxB, from C. botulinum strain okra, all protein sequences from the complex except that of HA-70 were found to match proteins known from other type B strains. It was found that matrix-assisted laser desorption ionisation MS provides provisional identification from trypsin digest peptide maps and that liquid chromatography electrospray (tandem) mass spectrometry affords unequivocal identification from amino acid sequence information of digest peptides obtained in trypsin or pepsin digestion. © 2002 Elsevier Science B.V. All rights reserved.
Molecular Sequence Numbers: D67030, L42537, M30196, M81186, U24431, U63808, X52066, X79102, X79104, X92973, Y13630, Y14239;
Chemicals/CAS: Botulinum Toxin Type A; botulinum toxin type B; Botulinum Toxins; Pepsin A, EC 3.4.23.1; Trypsin, EC 3.4.21.4
Molecular Sequence Numbers: D67030, L42537, M30196, M81186, U24431, U63808, X52066, X79102, X79104, X92973, Y13630, Y14239;
Chemicals/CAS: Botulinum Toxin Type A; botulinum toxin type B; Botulinum Toxins; Pepsin A, EC 3.4.23.1; Trypsin, EC 3.4.21.4
Topics
Amino acid sequencingBotulinum toxinsMass spectrometryPeptide mappingToxinsAmino acidsBacteriaDesorptionIonizationLaser applicationsMass spectrometryMilitary operationsProteinsElectrosprayToxic materialsBotulinum toxin ABotulinum toxin BHemagglutininPepsin ATrypsinAmino acid sequenceAnalytic methodArticleBacterial strainClostridium botulinumData baseGene sequenceLiquid chromatographyMass spectrometryPriority journalProtein analysisSequence alignmentAmino Acid SequenceBotulinum Toxin Type ABotulinum ToxinsMolecular Sequence DataPepsin ASequence Homology, Amino AcidSpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTrypsinAbelmoschusBacteria (microorganisms)ClostridiumClostridium botulinum
TNO Identifier
236690
ISSN
0021-9673
Source
Journal of Chromatography A, 970(1-2), pp. 95-115.
Pages
95-115
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