A neoantigenic determinant in the D-dimer fragment of fibrin

article






Lugovskoy, E.V.

Kolesnikova, I.N.

Gritsenko, P.G.

Zolotareva, E.N.

Gaffney, P.

Nieuwenhuizen, W.

Komisarenko, S.V
Monoclonal antibody (mAb) III-3b binds D-dimer with Kd = 1.4 × 10-10 M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bβ134-190, presumably in Bβ155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant in D-dimer upon plasmic lysis of fibrin. mAb III-3b may be used as a tool for immunodiagnostic quantification of D-dimer in blood plasma. © 2002 Elsevier Science Ltd. All rights reserved. Chemicals/CAS: Antibodies, Monoclonal; Epitopes; Fibrin Fibrinogen Degradation Products; fibrin fragment D; Fibrin, 9001-31-4
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D-dimerFibrinMonoclonal antibodyNeoantigenic determinantD dimerepitopefibrinfibrinogenmonoclonal antibodymonoclonal antibody III dbunclassified drugfibrin degradation productfibrin fragment dantigen bindingarticleblood analysiscontrolled studycross reactiondissociation constantenzyme linked immunosorbent assayhumanimmunoblottingmethylationpriority journalprotein isolationserodiagnosisantibody affinityepitope mappingimmunologyAntibodies, MonoclonalAntibody AffinityEnzyme-Linked Immunosorbent AssayEpitope MappingEpitopesFibrinFibrin Fibrinogen Degradation ProductsHumansImmunoblotting
TNO Identifier
236652
Repository link
https://resolver.tno.nl/uuid:a6df298d-b648-42f2-9a8c-78fd00f11be9
ISSN
00493848
Source
Thrombosis Research, 107(3-4), pp. 151-156.
Pages
151-156
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