One repeat of the cell wall binding domain is sufficient for anchoring the Lactobacillus acidophilus surface layer protein

article

Smit, E.

Pouwels, P.H.
The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding. Apparently, SAC1 is necessary and sufficient for cell wall binding. Our data suggest that SAC anchors the S-protein to a cell wall teichoic acid. Chemicals/CAS: Bacterial Proteins; Disinfectants; Membrane Glycoproteins; Membrane Proteins; Phenol, 108-95-2; surface array protein, bacteria
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Cell membrane proteinSac1 proteinUnclassified drugVitronectinAmino terminal sequenceBacterial cell wallBacterial geneticsBacterium adherenceCarboxy terminal sequenceControlled studyNonhumanProtein domainBacterial ProteinsCell WallDisinfectantsLactobacillus acidophilusMembrane GlycoproteinsMembrane ProteinsPhenolProtein BindingProtein Structure, TertiarySonicationActinobacteria (class)Bacteria (microorganisms)LactobacillusLactobacillus acidophilusUncultured actinomycete
TNO Identifier
236651
Repository link
https://resolver.tno.nl/uuid:29d1cca8-59cf-42d5-9718-9364976c3681
ISSN
00219193
Source
Journal of Bacteriology, 184(16), pp. 4617-4619.
Pages
4617-4619
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