Retrospective detection of exposure to organophosphorus anti-cholinesterases: mass spectrometric analysis of phosphylated human butyrylcholinesterase

article






Fidder, A.

Hulst, A.G.

Noort, D.

Ruiter, R. de

Schans, M.J. van der

Benschop, H.P.

Langenberg, J.P.
In this paper a novel and general procedure is presented for detection of organophosphate-inhibited human butyrylcholinesterase (HuBuChE), which is based on electrospray tandem mass spectrometric analysis of phosphylated nonapeptides obtained after pepsin digestion of the enzyme. The utility of this method is exemplified by the positive analysis of serum samples from Japanese victims of the terrorist attack with sarin in the Tokyo subway in 1995.
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Cholinesterase inhibitorNerve gasOrganophosphorus compoundPronaseColorimetryEnzyme activityEnzyme assayHigh performance liquid chromatographyHydrolysisIntoxicationMass spectrometryProtein phosphorylationSpectrometrySynthesisButyrylcholinesteraseEnvironmental ExposureEnvironmental MonitoringHumansInsecticidesPeptide FragmentsElectrospray IonizationTerrorismButyrylcholinesterase, EC 3.1.1.-Peptide fragmentsSarin, 107-44-8Warfare
TNO Identifier
236527
Repository link
https://resolver.tno.nl/uuid:7db2708a-2e28-43e4-aad0-bac2b4241d1c
ISSN
0893-228X
Source
Chemical Research in Toxicology, 15(4), pp. 582-590.
Pages
582-590
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