Cytotoxicity of dental composite components and mercury compounds in lung cells
article
Objective: The effect of dental composite components triethyleneglycoldimethacrylate (TEGDMA) and hydroxyethylmethacrylate (HEMA), as well as mercuric chloride (HgCl2) and methylmercury chloride (MeHgCl) was investigated on the release of lactatedehydrogenase (LDH) from alveolar epithelial lung cell lines in vitro. Methods: The confluent cell layers from the A549 (human, malignant) and the L2 cells (rat) were incubated with various concentrations of HEMA, TEGDMA, MeHgCl and HgCl2 at 37°C in 2% (v/v) CO2 atmosphere for 8 h. In further experiments the L2 cells were incubated with the same compounds for 6-48 h. LDH release was measured and the values were expressed as percentage of the LDH content. The values were plotted on a concentration log-scale and the substance concentration at the maximum slope was assessed as effective concentration (EC50). Results: A significant (p < 0.05) increase in the LDH release was found in the L2 cells after 8-h incubation with HEMA (4 mmol/1), TEGDMA (2 mmol/l), MeHgCl (0.01 mmol/l) and HgCl2 (0.015 mmol/l), and in A549 cells with HEMA (14 mmol/l), TEGDMA (15 mmol/l), MeHgCl (0.15 mmol/l) and HgCl2 (0.05 mmol/l), compared to controls. The EC50 values from compounds in the L2 cells are shown in the following table (mean; sem in parentheses; n = 3-6; #n = 1): Time (h) 6 12 24 36 48 HEMA 5.46 (1.61) 4.66 (1.97) 3.68 (1.15) 3.22 (1.38) 0.59 (0.17) TEGDMA 3.37 (1.65) 1.30 (0.39) 1.47 (0.33) 1.58 (0.28) 0.42 (0.10) MeHgCl 0.018 (0.004) 0.007 (0.001) 0.009 (0.000) 0.009 (0.000) 0.003 (0.002) HgCl2 0.016 (0.000) 0.014 (0.000) 0.009 (0.003) 0.008 (0.004) 0.007# Significance: The toxic effect of HgCl2 and MeHgCl from the L2 cells was about 100-700-fold higher than of the dental composite components. A significant (p < 0.05) time dependent increase of toxicity was observed with TEGDMA, HEMA and MeHgCl. © 2001 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.
Topics
CytotoxicityDental materialHEMALactatedehydrogenaseLung cellsMercuric chlorideMethylmercury chlorideTEGDMA2 hydroxyethyl methacrylateBiomaterialBiomedical and dental materialsLactate dehydrogenaseMacrogol derivativeMercury derivativeMethacrylic acid derivativeMethylmercuric chlorideMethylmercury derivativePolymethacrylic acid derivativeResinTopical antiinfective agentTriethylene glycol dimethacrylateAnimalCell cultureCell lineCytologyDrug effectEpithelium cellHumanLungLung alveolusLung tumorPathologyRatStatistical modelStatisticsTemperatureTimeAnti-infective agents, localBiocompatible materialsCell lineComposite resinsDental materialsEpithelial cellsLinear modelsMercury CompoundsMethacrylatesMethylmercury compoundsPolyethylene glycolsPolymethacrylic acidsPulmonary alveoliTime factorsTumor cells, CulturedHydroxyethyl methacrylate, 868-77-9L-Lactate Dehydrogenase, EC 1.1.1.27Mercuric chloride, 7487-94-7Methylmercuric chloride, 115-09-3Triethylene glycol dimethacrylate, 109-16-0
TNO Identifier
235994
Source
Dental Materials, 17(2), pp. 95-101.
Pages
95-101
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