Determination of alkylphosphonic acids by microcolumn liquid chromatography with gradient elution coupled on-line with flame photometric detection

article


Hooijschuur, E.W.J.

Kientz, C.E.

Brinkman, U.A.T.
Microcolumn liquid chromatography with gradient elution and on-line flame photometric detection has been used for the selective and rapid determination of non-volatile alkylphosphonic acids, which are hydrolysis products of nerve agents, in aqueous samples. A make-up of water was used to maintain stable introduction of the eluent during a gradient run. The detection limits are strongly dependent on quenching, which is determined by the mass flow of methanol in the eluent. Large-volume injections of 100 μl of aqueous solutions of short- and long-chain alkylphosphonic acids resulted in detection limits in the range 6-800 ng/ml. The repeatability of the retention times and analyte response - peak area or peak height - were 0.7-0.9% and 4-11%, respectively. The method was successfully applied to a local tap water sample and an aqueous soil extract. © 2001 Elsevier Science B.V.
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Alkylphosphonic acidsDetection, LCFlame photometric detectionLarge-volume injectionsWarfare agentsAlkyl groupPhosphonic acid derivativeChemical analysisChemical warfareFlame photometryHydrolysisLiquid chromatographyMolecular stabilityReproducibilityChromatography, LiquidPhosphonic AcidsPhotometry
TNO Identifier
235952
Repository link
https://resolver.tno.nl/uuid:d6187120-3303-44e5-96e5-439b912f2209
Source
Journal of Chromatography A, 907(1-2), pp. 165-172.
Pages
165-172
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