14-3-3 proteins interact with a 13-lipoxygenase, but not with a 9-lipoxygenase
article
Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development. Copyright (C) 2000 Federation of European Biochemical Societies. Molecular Sequence Numbers: GENBANK: L35931, L37358, X62388, X93170, Y14200;
Chemicals/CAS: 13-lipoxygenase, EC 1.13.11.-; 14-3-3 Proteins; Antibodies, Monoclonal; Isoenzymes; Lipoxygenase, EC 1.13.11.12; Proteins; Recombinant Proteins; Tyrosine 3-Monooxygenase, EC 1.14.16.2
Chemicals/CAS: 13-lipoxygenase, EC 1.13.11.-; 14-3-3 Proteins; Antibodies, Monoclonal; Isoenzymes; Lipoxygenase, EC 1.13.11.12; Proteins; Recombinant Proteins; Tyrosine 3-Monooxygenase, EC 1.14.16.2
Topics
Interaction13 lipoxygenase9 lipoxygenaselipoxygenaseunclassified drugbarleyenzyme purificationenzyme substrate complexgerminationimmunoprecipitationnucleotide sequenceprotein familyprotein phosphorylationsurface plasmon resonanceWestern blotting14-3-3 ProteinsAntibodies, MonoclonalChemistry, PhysicalEscherichia coliHordeumImmunosorbent TechniquesIsoenzymesLipoxygenaseProteinsRecombinant ProteinsSurface Plasmon ResonanceTyrosine 3-MonooxygenaseHordeum vulgare subsp. vulgare
TNO Identifier
235560
ISSN
00145793
Source
FEBS Letters, 474(1), pp. 48-52.
Pages
48-52