Synthesis of lactococcin 972, a bacteriocin produced by Lactococcus lactis IPLA 972, depends on the expression of a plasmid-encoded bicistronic operon
article
Synthesis of lactococcin 972 is plasmid-encoded. An operon composed of two genes that encode pre-bacteriocin and a putative immunity protein has been identified. The first gene encodes a 91-residue polypeptide that is exported via a sec-dependent system to give the mature 66-aa bacteriocin. The immunity protein is a 563-residue polypeptide with seven potential transmembrane domains. Two transcripts were observed from this region: one comprises the whole operon and is synthesized during the exponential phase of growth while the other, which corresponds just to the bacteriocin structural gene, presents a maximum in exponential cultures but is still present in late-stationary-phase cells.
Topics
BacteriocinLactococcin 972Lactococcus lactisPlasmid-encoded genesLactococcinBacterial geneGene expressionLactococcus lactisNonhumanNucleotide sequenceOperonPlasmidPriority journalProtein synthesisStructural geneAmino Acid SequenceBacteriocinsBase SequenceBlotting, NorthernElectrophoresis, Polyacrylamide GelGenes, BacterialLactococcus lactisMolecular Sequence DataOperonPlasmidsRNA, BacterialTime Factors
TNO Identifier
235201
ISSN
13500872
Source
Microbiology, 145(11), pp. 3155-3161.
Pages
3155-3161
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