The β-adrenoceptor agonist clenbuterol is a potent inhibitor of the LPS-induced production of TNF-α and IL-6 in vitro and in vivo
article
Objective and Design: To investigate the suppressive effects of the β-agonist clenbuterol on the release of TNF-α and IL-6 in a lipopolysaccharide (LPS)-model of inflammation, both in vitro and in vivo. Material and Subjects: Human U-937 cell line (monocyte-derived macrophages), and male Wistar rats (200-250 g). Treatment: U-937 macrophages were incubated with LPS at 1 μg/ml, with or without 1.0 mM-0.1 nM test drugs (clenbuterol and other cAMP elevating agents) for 1-24 h. Rats were administered either 1 or 10 μg/kg clenbuterol (or saline) orally, 1 h before intraperitoneal administration of 2 mg/kg LPS. Methods and Results: TNF-α and IL-6 time-concentration profiles were determined both in culture media and plasma, using ELISA's and bioassays. LPS-mediated release of both cytokines was significantly suppressed by clenbuterol. Conclusions: The β-agonist clenbuterol very potently suppresses the LPS-induced release of the pro-inflammatory cytokines TNF-α and IL-6 both in vitro and in vivo.
Topics
β-agonistsClenbuterolCytokinesLipopolysaccharideMacrophagesBeta adrenergic receptor stimulating agentClenbuterolInterleukin 6LipopolysaccharideTumor necrosis factor alphaAnimal cellBioassayBlood levelControlled studyCulture mediumCytokine productionEnzyme linked immunosorbent assayHumanHuman cellInflammationMacrophageMaleMonocyteNonhumanOral drug administrationRatAdrenergic beta-AgonistsAnimalsCell LineClenbuterolEscherichia coliHumansInterleukin-6LipopolysaccharidesMacrophagesMaleRatsRats, WistarTumor Necrosis Factor-alpha
TNO Identifier
235186
ISSN
10233830
Source
Inflammation Research, 48(9), pp. 497-502.
Pages
497-502
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