Development of safe oral vaccines based on Lactobacillus as a vector with adjuvanticity
article
Single dose live oral vaccines are most urgently needed to meet the prerequisites as phrased in the `Children's Vaccine Initiative'. The use of a Generally Regarded as Safe (GRAS) micro-organism as Lactobacillus as oral vaccine carrier has the advantage a.o. that these bacteria have probiotic, health beneficial properties and can be used as `food-grade' delivery vehicles. In mice, using model antigens we have investigated the properties of various Lactobacillus strains as a carrier and as an adjuvant. It was shown that some Lactobacillus-strains do possess adjuvant properties to a level as observed with well established water-in-oil adjuvants whereas other strains do not provide immuno-enhancing effects. Haptenated Lactobacillus demonstrated the carrier function which is provided in serum antibody as well as DTH responses. Synthetic peptides with at least one B-cell and one T-cell epitope coupled to the surface of the bacteriae indicated that surface expression best supports immune response induction (antibodies, DTH). Expression vectors for Lactobacillus with surface anchored viral- and bacterial peptide-antigens as well as vectors allowing the intracellular expression and secretion of antigens have been developed and are being tested in vaccine feasibility studies.
Topics
TNO Identifier
86950
ISSN
03070492
Source
Proceedings ICHEM, 2, pp. 43-46.
Pages
43-46
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