HLA-class II-associated control of antigen recognition by T cells in leprosy: A prominent role for the 30/31-kDa antigens
article
The recognition of 16 mycobacterial Ags by a panel of T cell lines from leprosy patients and healthy exposed individuals from an endemic population was examined within the context of expressed HLA-DR molecules. Although overall no significant differences were found between the frequencies of Ag recognition in the different subject groups, when Ag-specific T cell responses were examined within the context of HLA-DR, a highly significant difference was found in the recognition of the 30/31-kDa Ag. HLA-DR3 appeared to be associated with high T cell responsiveness to the 30/31-kDa Ag in healthy contacts (p = 0.01), but, conversely, with low T cell responsiveness to this Ag in tuberculoid patients (p = 0.005). Within the group of HLA-DR3- positive individuals, differences in 30/31-kDa directed T cell responsiveness were highly significant not only between healthy individuals and tuberculoid patients (p < 0.0001), but also between healthy individuals and lepromatous patients (p = 0.009), and consequently between healthy individuals compared with leprosy patients as a group (p < 0.0001). A dominant HLA-DR3-restricted epitope was recognized by healthy contacts in this population. It has been proposed that secreted Ags may dominate acquired immunity early in infection. The low T cell response to the secreted, immunodominant 30/31-kDa Ag in HLA- DR3-positive leprosy patients in this population may result in retarded macrophage activation and delayed bacillary clearance, which in turn may lead to enhanced Ag load followed by T cell-mediated immunopathology. Chemicals/CAS: Antigens, Bacterial; Epitopes, T-Lymphocyte; HLA-DR Antigens; HLA-DR3 Antigen
Topics
EpitopeHLA antigen class 2HLA DP antigenMycobacterium antigenAntigen recognitionAntigen specificityCell lineCellular immunityControlled studyHuman cellLepromatous leprosyLeprosyMacrophage activationMajor clinical studyTuberculoid leprosyAntigens, BacterialEpitope MappingEpitopes, T-LymphocyteHLA-DR AntigensHLA-DR3 AntigenHumansImmunophenotypingLeprosyLymphocyte ActivationMolecular WeightMycobacterium lepraeT-Lymphocytes
TNO Identifier
235044
ISSN
00221767
Source
Journal of Immunology, 162(11), pp. 6912-6918.
Pages
6912-6918
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