17α-Ethyl-5β-estrane-3α,17β-diol, a biological marker for the abuse of norethandrolone and ethylestrenol in slaughter cattle

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Puymbroeck, M. van

Kuilman, M.E.M.

Maas, R.F.M.

Witkamp, R.F.

Leyssens, L.

Miert, A.S.J.P.A.M. van

Hendriks, L.

Zande, D.J.M. van der

Adriaensens, P.

Jacobs, M.P.

Raus, J.
The metabolism of the illegal growth promoter ethylestrenol (EES) was evaluated in bovine liver cells and subcellular fractions of bovine liver preparations. Incubations with bovine microsomal preparations revealed that EES is extensively biotransformed into norethandrolone (NE), another illegal growth promoter. Furthermore, incubations of monolayer cultures of hepatocytes with NE indicated that NE itself is rapidly reduced to 17α-ethyl-5β-estrane-3α,17β-diol (EED). In vivo tests confirmed that, after administration of either EES or NE, EED is excreted as a major metabolite. Therefore, it was concluded that, both in urine and faeces samples, EED can be used as a biological marker for the illegal use of EES and/or NE. Moreover, by monitoring EED in urine or faeces samples, the detection period after NE administration is significantly prolonged. These findings were further confirmed by three cases of norethandrolone abuse in a routine screening program for forbidden growth promoters. Copyright (C) 1999 Elsevier Science B.V.
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17α-Ethyl-5β-estrane-3α,17β-diolEthylestrenolNorethandroloneSteroidsBiological markerEthylestrenolNorethandrolone17Alpha ethyl 5 xi estrane 3 xi,17 beta diolDrug derivativeDrug residueEstradiolAnimal experimentAnimal tissueArticleBiotransformationCattleDrug abuseDrug metabolismDrug monitoringFecesFemaleIntramuscular drug administrationLiverLiver cell cultureMaleNonhumanPriority journalReductionUrineAnimalAnimal husbandryChemistryHigh performance liquid chromatographyLiver microsomeMass fragmentographyNuclear magnetic resonance spectroscopySensitivity and specificityStandardThin layer chromatographyUltraviolet spectrophotometryBiological MarkersCattleChromatography, High Pressure LiquidChromatography, Thin LayerDrug ResiduesEstradiolEthylestrenolGas Chromatography-Mass SpectrometryMagnetic Resonance SpectroscopyMaleMicrosomes, LiverNorethandroloneReference StandardsSensitivity and SpecificitySpectrophotometry, Ultraviolet
TNO Identifier
235039
Repository link
https://resolver.tno.nl/uuid:e360a7ec-7e59-4bea-89f1-7a1d977446a0
ISSN
13872273
Source
Journal of Chromatography B: Biomedical Sciences and Applications, 728(2), pp. 217-232.
Pages
217-232
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