Capillary electrophoresis coupled on-line with nitrogen-selective thermionic detection using an eluent-jet interface
article
The on-line coupling of capillary electrophoresis (CE) with a thermionic detector (TID) originally developed for gas chromatography (GC) is reported for the first time. An eluent-jet interface previously used to combine micro- liquid chromatography and mass spectrometry can now be used to couple CE and TID. Preliminary results demonstrate the possibility of performing direct nitrogen-selective detection, even for less volatile compounds. Detection limits of 4-12 pg N/s were obtained for the four tested compounds piperidine, dimethylformamide, tetra-n-butylammonium hydroxide, and nicotinic acid. Optimization of both the TID and the eluent-jet interface can be performed independently; optimum TID parameters agree rather closely with the detector specifications under GC conditions. The nitrogen-selective TID proved to be linear over more than three orders of magnitude with acceptable repeatability. The on-line coupling of CE and TID may well become an interesting approach for the rapid and direct analysis of ionic and high- molecular-mass N-containing compounds.
Topics
TNO Identifier
234316
ISSN
0935-6304
Source
HRC Journal of High Resolution Chromatography, 21(10), pp. 540-544.
Publisher
Wiley
Pages
540-544
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