Removal of UV-induced DNA lesions in mouse epidermis soon after irradiation
article
Induction and removal of cyclobutane thymine dimers and (6-4)photoproducts were studied in epidermal DNA isolated from UV-exposed hairless mice. For the detection of DNA damage, lesion-specific monoclonal antibodies were used in an immunoslotblot assay. Following the exposure of mice to 3.0 kJ m-2 UV-B, substantial removal of both thymine dimers (66%) and (6-4)photoproducts (77%) was observed at 24 h after irradiation. No removal, however, was detected at 4 h after irradiation. In contrast, immunofluorescence data obtained previously showed a rapid initial dimer removal after irradiation with 1.0.kJ m-2 UV-B (A.A. Vink, R.J.W. Berg, F.R. De Gruijl, L. Roza and R.A. Baan, Carcinogenesis l2 (1991) 861-64.) Reinvestigation of the removal of dimers and (6-4)photoproducts shortly after three different W doses showed a rapid decrease of both lesions at 2 h after irradiation with 1.0 kJ m-2. The results obtained after irradiations with 2.0 and 3.0 kJ m-2 UV-B suggest a saturation of repair already at 2.0 kJ m-2. Cyclobutane dimers were found to be removed at a lower rate than (6-4)photoproducts. Chemicals/CAS: cyclobutane, 287-23-0; DNA, 9007-49-2; Pyrimidine Dimers
Topics
(6-4)PhotoproductsCyclobutane thymine dimersHairless miceImmunoblot assayUV-BCyclobutaneMonoclonal antibodyAnimal experimentAnimal modelControlled studyHela cellHuman cellImmunofluorescenceMouseNonhumanUltraviolet irradiationAnimalDNADNA DamageDNA RepairDose-Response Relationship, RadiationEpidermisFemaleHela CellsHumanImmunoblottingKineticsMiceMice, Inbred HRSPyrimidine DimersSupport, Non-U.S. Gov'tTime FactorsUltraviolet Rays
TNO Identifier
82150
Source
Journal of Photochemistry and Photobiology B : Biology, 24, pp. 25-31.
Pages
25-31
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