Accurate determination of process variables in a solid-state fermentation system
article
The solid-state fermentation (SSF) method described enabled accurate determination of variables related to biological activity. Growth, respiratory activity and production of carboxymethyl-cellulose-hydrolysing enzyme (CMC-ase) activity by Trichoderma reesei QM9414 on wheat bran was used as a model SSF. The standard deviation (s) of measured substrate weight loss and glucosamine content, and the overall standard deviation of oxygen consumption rate (OCR) and carbon-dioxide production rate (CPR) after 72 h of fermentation were less than 7% of the mean measured values. A statistical method to estimate the standard deviation of time-dependent data is presented. A closed carbon balance could be set up with the results of the measurements of CPR, elemental composition and chemical composition, indicating the reliability of the methods used. Measurements of CMC-ase activity were less accurate due to interaction between substrate and product. The measurement of the ATP level was also less accurate.
Topics
TNO Identifier
233608
ISSN
13595113
Source
Process Biochemistry, 31(7), pp. 669-678.
Pages
669-678
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