Isolation and storage of DNA for population studies
article
For genetic population studies, human genomic DNA is commonly isolated from peripheral blood. A fast, non-invasive DNA sampling method is developed involving oral samples taken with cotton swabs. In addition various procedures were compared for isolation of DNA from different sources: whole blood or buffy-coats stored at -20°C for 5-10 years or buccal cells collected freshly with the non-invasive method. The differences in these procedures, which do not contain a phenol-extraction, are based on the use of either 1) high concentration ammonium acetate followed by DNA precipitation or 2) high concentration potassium acetate, followed by chloroform extraction and normal DNA precipitation.
TNO Identifier
233522
ISSN
02689499
Source
Fibrinolysis, 10(SUPPL. 2), pp. 29-30.
Pages
29-30
Files
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