The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA
article
S-proteins are proteins which form a regular structure (S-layer) on the outside of the cell walls of many bacteria. Two S-protein-encoding genes are located in opposite directions on a 6.0-kb segment of the chromosome of Lactobacillus acidophilus ATCC 4356 bacteria. Inversion of this chromosomal segment occurs through recombination between two regions with identical sequences, thereby interchanging the expressed and the silent genes. In this study, we show that the region involved in recombination also has a function in efficient S-protein production. Two promoter sequences are present in the S-protein gene expression site, although only the most downstream promoter (P-1) is used to direct mRNA synthesis. S-protein mRNA directed by this promoter has a half-life of 15 min. Its untranslated leader can form a stable secondary structure in which the 5' end is base paired, whereas the ribosome- binding site is exposed. Truncation of this leader sequence results in a reduction in protein production, as shown by reporter gene analysis of Lactobacillus casei. The results obtained indicate that the untranslated leader sequence of S-protein mRNA is involved in efficient S-protein production.
Topics
Bacterial virulenceEpithelium cellGene expression regulationGenetic analysisGenetic recombinationNonhumanPriority journalPromoter regionProtein secondary structureProtein stabilityRibosome subunitRna sequenceRna transcriptionBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceCell WallConsensus SequenceGene ExpressionGene Expression Regulation, BacterialLactobacillus acidophilusLactobacillus caseiMembrane GlycoproteinsMembrane ProteinsMolecular Sequence DataNucleic Acid ConformationPromoter Regions (Genetics)Recombination, GeneticRNA, BacterialRNA, MessengerSpecies SpecificityTranscription, GeneticBacteria (microorganisms)
TNO Identifier
233470
ISSN
00219193
Source
Journal of Bacteriology, 178(18), pp. 5388-5394.
Pages
5388-5394
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