Medium optimization for nuclease P1 production by Penicillium citrinum in solid-state fermentation using polyurethane foam as inert carrier
article
A solid-state fermentation system, using polyurethane foam as an inert carrier, was used for the production of nuclease P1 by Penicillium citrinum. Optimization of nuclease P1 production was carried out using a synthetic liquid medium. After a two-step medium optimization using a fractional factorial experimental design, nuclease P1 productivity and specific activity are greatly increased. Nuclease P1 productivity and specific activity are ninefold and fourfold higher, respectively, than in solid-state fermentation of wheat bran and 500-fold and 20-fold higher, respectively, than in submerged fermentation. Additional advantages of the solid-state fermentation process using inert carrier are discussed. A solid-state fermentation system, using polyurethane foam as an inert carrier, was used for the production of nuclease P1 by Penicillium citrinum. Optimization of nuclease P1 production was carried out using a synthetic liquid medium. After a two-step medium optimization using a fractional factorial experimental design, nuclease P1 productivity and specific activity are greatly increased. Nuclease P1 productivity and specific activity are ninefold and fourfold higher, respectively, than in solid-state fermentation of wheat bran and 500-fold and 20-fold higher, respectively, than in submerged fermentation. Additional advantages of the solid-state fermentation process using inert carrier are discussed.
Topics
Inert carrierMedium optimizationNuclease P1Penicillium citrinumPolyurethane foamSolid-state fermentationBiotechnologyFermentationFoamed plasticsMicroorganismsOptimizationPolyurethanesFractional factorial experimental designInert carrierNuclease P1Penicillium citrinumPolyurethane foamSolid state fermentationWheat branEnzymesNucleasePolyurethan foamArticleCulture mediumEnzyme activityEnzyme synthesisFermentationMethodologyNonhumanPenicilliumWheat bran
TNO Identifier
233207
ISSN
01410229
Source
Enzyme and Microbial Technology, 18(2), pp. 108-112.
Pages
108-112
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