A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium

article



Argnani, A.

Leer, R.J.

Luijk, N. van

Pouwels, P.H.
A protocol was developed for the introduction of foreign plasmid DNA into various Bifidobacterium strains. The method, which is applicable to all Bifidobacterium species tested so far, is based on electroporation of bacteria made competent by preincubation in electroporation buffer for several hours at 4°C. Transformation of Bifidobacterium could be achieved with a plasmid vector originating from Bifidobacterium and with plasmid vectors from Corynebacterium, but not with vectors carrying replicons from Lactococcus or Lactobacillus.
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ElectrotransformationPlasmid vectorPlasmid dnaBacterium competenceVontrolled studyExpression vectorGenetic transformationLactobacillus bifidusMethodologyNonhumanPriority journalAmino Acid SequenceBifidobacteriumCorynebacteriumDNA ReplicationElectroporationGenes, BacterialGenetic VectorsLactobacillusLactococcusMolecular Sequence DataPlasmidsReproducibility of ResultsSequence Homology, Amino AcidSpecies SpecificityTransformation, Genetic
TNO Identifier
233155
Repository link
https://resolver.tno.nl/uuid:84f3f325-ae7e-4e86-8ba0-c0bc94af1bac
ISSN
13500872
Source
Microbiology, 142(1), pp. 109-114.
Pages
109-114
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