The region Ser333-Arg356 of the alpha-chain of human C4b-binding protein is involved in the binding of complement C4b
article
Human C4b-binding protein (C4BP) functions as a cofactor to factor I in the degradation of C4b and accelerates the decay rate of the C4b2a complex. In this study we describe a monoclonal antibody directed against the α-chain of C4BP that inhibits the binding of C4b to C4BP. In order to identify the structural domain of the α-chain of C4BP that interacts with C4b, tryptic fragments of C4BP were generated. Amino acid sequence analysis of the fragments revealed that the residues Ser333-Arg356 of the α-chain of C4BP contain the epitope of this antibody, and as a consequence, that this part of the α-chain of C4BP is likely to be involved in the interaction with C4b. Chemicals/CAS: complement component C4b, 80295-50-7; Antibodies, Monoclonal; Carrier Proteins; Complement 4b, 80295-50-7; complement 4b-binding protein; Receptors, Complement
Topics
TNO Identifier
80343
ISSN
00145793
Source
FEBS Letters, 317(3), pp. 228-232.
Pages
228-232